M.Sc. in Molecular Biology and Biotechnology

 M.Sc. in Molecular Biology and Biotechnology

Molecular Biology and Biotechnology

TRIMESTER WISE DISTRIBUTION OF COURSES

 

I TRIMESTER             

• PRINCIPLES OF BIOTECHNOLOGY         
• FUNDAMENTALS OF MOLECULAR BIOLOGY     
• MOLECULAR CELL BIOLOGY        
• MOLECULAR GENETICS    
• IMMUNOLOGY AND MOLECULAR DIAGNOSTICS          
• BIOTECHNOLOGY LAB
• SEMINAR

 

II TRIMESTER  

• PLANT TISSUE CULTURE AND GENETIC TRANSFORMATION
• BIOINFORMATICS
• BIOTECHNOLOGY-II
• MOLECULAR BREEDING   
• GENOMICS  
• SEMINAR      
• ADVANCES IN PLANT MOLECULAR BIOLOGY

 

III TRIMESTER  

• BIOSAFETY, IPR AND BIOETHICS
• INDUSTRIAL BIOTECHNOLOGY    
• BIOTECHNOLOGY LAB-III  
• SEMINAR      
• ADVANCES IN GENETIC ENGINEERING 
• ADVANCES IN MOLECULAR BREEDING AND GENOMICS
• ADVANCES IN STRESS GENOMICS         

 

Core Courses

• M.Sc.: MBB 501, MBB 502, MBB 504, MBB 505, MBB 510, MBB 511, MBB 512, BIO 501, GP 500
• Ph.D.: MBB 601, MBB 602

 

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MOLECULAR BIOLOGY AND BIOTECHNOLOGY

• Major Field : MBB for M.Sc. and Ph.D.
• Minor Field : Ph.D. students shall take two minors (9 credits of coursework in each) from any of the other fields outside his/her own. M.Sc. students shall take one minor (9 credits of coursework) from any of the other fields outside his/her own discipline.

 

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DESCRIPTION OF COURSES

 

PRINCIPLES OF BIOTECHNOLOGY

Objective

• To provide insight into basics and application of general biotechnology

 

Theory

• UNIT I
• The structure of DNA; Function of genes and genomes; Restriction enzymes and vectors; Methods of recombinant DNA technology; Nucleic acid hybridization; PCR and its applications;
• UNIT II
• Molecular markers and their applications; DNA sequencing;
• UNIT III
• Applications of gene cloning in basic and applied research; Genomics, transcriptomics and proteomics;
• UNIT IV
• Genetic engineering and transgenics, General application of biotechnology in Agriculture, Medicine,
• Animal husbandry, Environmental remediation, Energy production and Forensics;
• UNIT V
• Public perception of biotechnology; Biosafety and bioethics issues; Intellectual property rights in biotechnology.

 

Suggested Readings

• Molecular biology (2005) by David P. Clark.
• Molecular biology of the Cell (2008) by Bruce Alberts.
• Molecular biology and Biotechnology (2009) by John M. Walker, Ralph Rapley Biotechnology: Expanding Horizons (2010) by B D Singh.

 

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FUNDAMENTALS OF MOLECULAR BIOLOGY

Objective

• To provide insight into fundamentals of molecular biology and study of molecular cell processes

 

Theory

• UNIT I
• Historical development of molecular biology; Nucleic acids as genetic material; chemistry and structure of DNA and RNA,
• UNIT II
• Genome organization in prokaryotes and eukaryotes; Chromatin structure and function,
• UNIT III
• DNA replication; DNA polymerases, topoisomerases, DNA ligase; Reverse transcriptase,
• UNIT IV
• Transcription process; RNA processing; RNA editing; Ribosomes; Structure and function; Organisation of ribosomal proteins and RNA genes,
• UNIT V
• Genetic code; Aminoacyl tRNA synthetases; Translation and post-translational modifications, Operon concept; Attenuation of trp operon

 

Suggested Readings

• Molecular genetics by (1987) Stent and Calendar
• Microbial Genetics (1994) by Stanley R. Maloy, David Freifelder, John E. Cronan
• Essential molecular biology: a practical approach (2000) by T. A. Brown
• Genes (2008) by B Lewine
• Molecular biology of the cell (2008) by Bruce Alberts
• Molecular Biology of the Cell (2002) by Julian Lewis

 

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MOLECULAR CELL BIOLOGY

Objective

• To provide insight into fundamentals of cell structure, organization and function

 

Theory

• UNIT I
• General structure and constituent of cells; Similarities and distinction between plant and animal cells; Cell wall, cell membrane, cell surface related function.
• UNIT II
• Structure and function of major organelles: Nucleus, Chloroplasts, Mitochondria, Endoplasmic reticulum, Microbodies, Golgi apparatus, Vacuoles
• UNIT III
• Organellar genomes and their manipulation; Ribosome in relation to cell growth and cell division; Cyto-skeletal elements; Water, protein and ion transport;
• UNIT IV
• Trafficking of biomolecules, Cell division and regulation of cell cycle; Signal transduction mechanisms.

 

Suggested Readings

• Molecular Cell Biology (2007) Bruce Alberts
• Molecular Cell Biology & Solutions Manual (2007) by Harvey Lodish, Arnold Berk, Chris A. Kaiser, Monty Krieger, et al.
• Plant Physiology (2008) by Taiz and Zieger

 

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PLANT TISSUE CULTURE & GENETIC ENGINEERING

Objective

• To provide insight into principles of plant cell culture and genetic transformation

 

Theory

• UNIT I
• History of plant cell / tissue culture; Culture media; Various types of culture; Callus, suspension, nurse, root, meristem,In vitro differentiation, organogenesis, somatic embryogenesis; Molecular basis of plant organ differentiation;
• UNIT II
• Plant growth regulators; Mode of action, effects on in vitro culture and regeneration; In vitro storage organ formation;
• UNIT III
• Micropropagation; Anther / microspore culture; Somaclonal variation; In vitro mutagenesis, protoplast culture; Somatic cell genetics and somatic hybridization; Embryo culture/wide hybridization; In vitro fertilization; Unit V: In vitro germplasm conservation; Cryopreservation; Production of secondary metabolites through in vitro culture;
• UNIT IV
• Plant genetic engineering: Definition and scope, methods of plant transformation, vectors for plant transformation,
• Unit V
• Genetic and molecular analyses of transgenics, biosafety issues, testing of transgenics, regulatory procedures for commercial approval.

 

Suggested Readings

• Plant Tissue Culture: Theory and Practice (1996) by Bhojwani and Razdan
• Plant Tissue culture: A practical approach (1995) Dixon and Dixon
• Plant Tissue Culture, Development, and Biotechnology (2010) by Robert N. Trigiano and Dennis J. Gray

 

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MOLECULAR GENETICS

Objective

• To provide insight into molecular genetics of prokaryotes and eukaryotes organisms

 

Theory

• UNIT I
• Mendelian principles of inheritance, molecular genetic systems, genetic variation, mutation, physical, chemical and insertional mutagenesis, site-directed mutagenesis, recombination and repair, UNIT II
• Gene mapping and tagging, tag based gene isolation, fine structure analysis of genetic loci and complementation test, deletion mapping,
• UNIT III
• Organization of genes in prokaryaotes and eukaryotes, gene and genome evolution, identification of cis-regulatory elements, transacting factors and regulation of gene expression at different levels,
• UNIT IV
• Deciphering of genetic code, gene-protein colinearity, gene-enzyme relationship, molecular gene concept. Plasmids and their inheritance
• UNIT V
• Introduction of genetic markers, classification and comparison of markers, basis of DNA polymorphism, principles & applications of genome mapping and DNA fingerprinting, genome analysis, classical and modern methods, principles of structural and functional genomic approaches.

Suggested Readings

• Molecular Biology of the Cell (2008) by Alberts and Watson
• Recombinant DNA (2006) by J Watson
• Molecular Cell Biology (1999) by Lodish and Baltimore
• Essential molecular biology: a practical approach (2000) by T. A. Brown
• Molecular Biotechnology (2009) by Glick & Pasternack

 

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BIOSAFETY, IPR AND BIOETHICS     

Objective

• To provide insight into the biosafety aspects of GM crops and IPR related issues

 

Theory

• UNIT I
• Biosafety and Risk assessment issues; Health aspects; toxicology, allergenicity; Ecological aspects;
• UNIT II
• Regulations; National biosafety policy and law. The Cartagena Protocol on biosafety. The WTO and other international agreements; Cross border movement of germplasm; Risk management issues;
• UNIT III
• Monitoring strategies and methods for detecting transgenics; Risks, benefits and impacts of transgenics to human health, society and the environment; Biosafety and biohazards; general principles for the laboratory and environmental biosafety;
• UNIT IV
• Environment Impact Assessment; Gene flow in natural and artificial ecologies; Sources of gene escape; Ecological risks of genetically modified plants.
• UNIT V
• Implications of intellectual property rights on the commercialization of biotechnology products.

 

Suggested Readings

• Theory of general ethics (2006) by Warwick Fox
• Against Bioethics (2007) by J Baron
• Bioethics and Biosafety (2008) by M K Sateesh
• Bioethics (2008) by S. Ignacimuthu
• Intellectual Property: Private Rights, the Public Interest, and the Regulation of Creative Activity (2007) by Shubha Ghosh; Richard S. Gruner;Jay P. Kesan; Robert I. Reis

 

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IMMUNOLOGY AND MOLECULAR DIAGNOSTICS

Objective

• To provide insight into basic concepts of immunology, vaccine development, techniques for diagnostics and applications in agriculture and pharmaceuticals

 

Theory

• UNIT I
• History and scope of immunology; components of immune system:. organs, tissues and cells,
• UNIT II
• Immunoglobulins chemistry, structure and functions; molecular organisation of immunoglobulins and classes of antibodies, antibody diversity; antigens, haptens, antigens- antibody interactions; immune-regulation and tolerance; Allergies and hypersensitive response; immunodeficiency; vaccines;
• UNIT III
• Immunological techniques, Immunological application in plant science, monoclonal antibodies and their uses, molecular diagnostics.
• UNIT IV
• Introduction to the basic principles of molecular technology and techniques used in pathogen detection, Principles of ELISA and its applications in viral detection,
• UNIT V
• Basics and procedures of PCR, Real time PCR, PCR based and hybridization based methods of detection, microarrays based detection, multiplexing etc, detection of soil borne and seed born infections, transgene detection in seed, planting material and processed food, molecular detection of varietal impurities and seed admixtures in commercial consignments.

 

Suggested Readings

• Kuby Immunology (2006) by Thomas J. Kindt, Barbara A. Osborne, and Richard A. Golds
• Roitt’s Essential Immunology (2006) by Peter Delves, Seamus Martin, Dennis Burton, and Ivan Roitt.

 

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INDUSTRIAL BIOTECHNOLOGY

Objective

• To provide insight into the industrial application of biotechnology and development of various products in mass scale for broad applications

 

Theory

• UNIT I
• Introduction, scope and historical developments. Isolation, screening and genetic improvement (involving classical approaches) of industrially important organisms.
• UNIT II
• Primary metabolism products, production of industrial ethanol as a case study, Secondary metabolites, bacterial antibiotics and non ribosomal peptide antibiotics. Microbial enzymes, role in various industrial processes, production of fine chemicals for pharmaceutical industries. Biotransformations, Bio-augmentation with production of vitamin C as a case study.
• UNIT III
• Bioreactors, their design and types. Immobilized enzymes based bioreactors. Microencapsulation technologies for immobilization of microbial enzymes. Industrial biotechnology for pollution control, treatment of industrial and other wastes, biomass production involving single cell protein.
• UNIT IV
• Bioremediation of soil. Production of eco-friendly agricultural chemicals, bio-pesticides, bioherbicides, bio-fertilizers, bio-fuels, etc. Recombinant DNA technologies for microbial processes.
• UNIT V
• Strategies for development of industrial microbial strains with scale up production capacities. Metabolic pathway engineering of microbes for production of novel products for industry.

 

Suggested Readings

• Encyclopedia of industrial Biotechnology (2010) by M Flickinger
• Manual of industrial Microbiology and Biotechnology (2010) by M.Demain
• Industrial Biotechnology (2010) by Wim Soetaert and Erick J Vandamme
• Biofuels: Biotechnology, Chemistry, and Sustainable Development (2008) by David M. Mousdale

 

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BIOINFORMATICS

Objective

• To provide information on basic principles of computational biology and statistical tools used for data analysis

 

Theory

• UNIT I
• Basic molecular biology; introduction to the basic principles of structure/function analysis of biological molecules; genome analysis; different types and classification of genome databases (e.g. HTGS, DNA, Protein, EST, STS, SNPs, Unigenes etc.)
• UNIT II
• Statistical Techniques: MANOVA, Cluster analysis, Discriminant analysis, Principal component analysis, Principal coordinate analysis, Multidimensional scaling; Multiple regression analysis; Likelihood approach in estimation and testing; Resampling techniques – Bootstrapping and Jackknifing; Markov Models. Hidden Markov Models, Bayesian estimation and Gibbs sampling 
• UNIT III
• DNA sequence retrieval system, various DNA and protein sequence file formats, Basic concepts of similarity searching and sequence alignments, pair wise and multiple sequence alignments, DNA sequence analysis, different gene prediction models and gene annotation tools,
• UNIT IV
• Protein sequence analysis and structure prediction, comparative genome analysis, phylogenetic analysis, gene expression analysis tools, programming languages and their applications in bioinformatics.

 

Practicals

• Different types of databases and database search and retrieval, DNA and protein sequence analysis, Similarity searching and multiple alignments, Gene annotation, Phylogenetic analysis, Sequence analysis, Protein structure prediction, Analysis of microarray data, Programming languages in bioinformatics

 

Suggested Readings

• DNA and protein sequence analysis. A Practical Approach (1997) by Bishop M.J., Rawlings C.J. (Eds.)
• Bioinformatics Basics: Applications in Biological Science and Medicine (2005) By Hooman Rashidi, Lukas K. Buehler
• Bioinformatics in the Post-Genomic Era: Genome, Transcriptome, Proteome, and InformationBased Medicine (2004) By Jeffrey Augen
• Frontiers in Computational Genomics (2003) Edited by: Michael Y. Galperin and Eugene V. Koonin

 

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BIOTECHNOLOGY LAB-I

Objective

• To provide skills for routine and advanced experimentation techniques in plant molecular biology

 

Theory

• UNIT I
• Good lab practices, Growth of bacterial culture and preparation of growth curve
• UNIT II
• Biochemical techniques, Preparation of buffers and reagents, electrophoresis- agarose and PAGE (nucleic acids and proteins), Principle of centrifugation
• UNIT III
• Isolation of genomic and plasmid DNA from bacteria, Growth of lambda phage and isolation of phage DNA, Restriction digestion of plasmid and phage DNA
• UNIT IV
• Chromatographic techniques (TLC, Gel Filtration Chromatography, Ion exchange
• Chromatography, Affinity Chromatography)
• Suggested Readings
• Current Protocols in Molecular Biology (2002) Fred M Ausubel Molecular Cloning: A Laboratory Manual (2001) J Sambrook 

 

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BIOTECHNOLOGY LAB-II  

Objective

• To provide hands on experience and skills in general molecular and biochemical laboratory techniques

 

Theory

• UNIT I
• Isolation of plant DNA, spectrophotometric and gel quantitation, restriction digestion, agarose gel electrophoresis, hybridization, autradiograph development (conventional X-ray film and Phosphorimager)Dot blot analysis,Northern hybridization
• UNIT II
• Gene cloning and blue white selection.
• UNIT III
• PCR and optimization of factors affecting PCR.,Synthesis and cloning of cDNA and RT-PCR analysis
• UNIT IV
• Western hybridization
• UNIT V
• Molecular markers (RAPD, SSR, AFLP etc) and their analysis

 

Suggested Readings

• Methods in Biotechnology (2009) by John Walker
• Protein Methods (1996) by Daniel M. Bollag, Michael D. Rozycki, Stuart J. Edelstein
• Basic Methods in Protein Purification and Analysis: A Laboratory Manual (2008) Edited By Richard
• J. Simpson

 

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BIOTECHNOLOGY LAB-III

Objective

• To provide hands on experience in general laboratory techniques and high throughput data analysis

 

Theory

• UNIT I
• Real time PCR and interpretation of data
• UNIT II
• Case study of SSR markers (linkage map, QTL analysis etc),SNP identification and analysis Unit III
• Microarray studies and use of relevant software, Practical in the area of Proteomics (2D gels, mass spectrometry etc)
• UNIT IV
• RNAi (right from designing of construct to the phenotyping of the plant),Yeast 2-hybrid interaction
• UNIT V
• Immunology and molecular diagnostics: Ouchterlony double diffusion, Immunoprecipitation, Radial Immunodiffusion, Immunoelectrophoresis, Rocket Immunoelectrophoresis, Counter
• Current Immunoelectrophoresis, ELISA, Latex Agglutination, Immunohistochemistry

 

Suggested Readings

• Current Protocols in Molecular Biology (2002) Fred M Ausubel
• Molecular Cloning: A Laboratory Manual (2001) J Sambrook
• Baxevanis, A. D. & Ouellette, B. F. F. (eds).. Bioinformatics: A Practical Guide to the Analysis of Genes and Proteins, Methods of Biochemical Analysis, (2001) vol. 43, 2nd ed., New York: John Wiley & Sons, Inc.
• Gibas, C. & Jambeck, P.. Developing Bioinformatics Computer Skills. (2001) by Sebastopol, CA: O’Reilly & Associates, Inc
• Handbook of Proteins: Structure, Function and Methods, (2008) by Michael M. Cox , George N. Phillips, Jr.

 

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MOLECULAR BREEDING

Objective

• To provide insight into principles of plant breeding using molecular techniques

 

Theory

• UNIT I
• Principles of plant breeding; breeding methods for self and cross pollinated crops; heterosis breeding; limitations of conventional breeding; aspects of molecular breeding;
• UNIT II
• Development of sequence based molecular markers - SSRs and SNPs; advanced methods of genotyping;
• UNIT III
• Mapping genes for qualitative and quantitative traits; QTL mapping using structured populations; AB-QTL analysis; association mapping of QTL; fine mapping of genes/QTL; map based gene/ QTL isolation and development of gene based markers;
• UNIT IV
• Methods of assessing genetic diversity & germplasm characterization, DNA fingerprinting and its application
• UNIT V
• Allele mining by TILLING and Ecotilling; use of markers in plant breeding; Marker assisted selection (MAS) in backcross and heterosis breeding; transgenic breeding; foreground and background selection; MAS for gene introgression and pyramiding: MAS for specific traits with examples.

 

Suggested Readings

• Molecular biology and genomics (2007) by Cornel Mülhardt
• Essential molecular biology: a practical approach (2000)by T. A. Brown
• Genomes (2006) By TA Brown
• Plant Genotyping (2001) by R. J. Henry
• DNA markers (1997) by G. Caetano Anolles and P. M. Gresshoff

 

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GENOMICS

Objective

• To provide insight into the functional aspects of cell function by studying the genome as a whole with special emphasis on structural and functional genomics

 

Theory

• UNIT I
• Structural genomics: large fragment genomic libraries, physical mapping of genomes, genome sequencing, sequence assembly and annotation, comparative genomics
• UNIT II
• Functional genomics: DNA chips and their use in transcriptome analysis, qPCR, SAGE, MPSS 
• UNIT III
• Proteome analysis: protein structure and function, proteins as enzymes, protein purification, 2D mass spectrometry, metabolomics and iominics, Application of genomics in crop improvement

 

Suggested Readings

• Genomes (2006) by TA Brown
• Evolutionary Genomics and Systems Biology (2010) by Gustavo Caetano
• Principles of Gene Manipulation and Genomics (2006) by Sandy B. Primrose and Richard Twyman New and Emerging Proteomic Techniques (2010) by D. Nedelkov and R. Nelson
• Metabolomics: A Powerful Tool in Systems Biology (2009) by Jens Nielsen and Michael C. Jewett Plant Metabolomics (2006) by Kazuki Saito, Richard A. Dixon and Lothar Willmitzer

 

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ADVANCES IN PLANT MOLECULAR BIOLOGY  

Objective

• To provide in depth knowledge of recent developments of plant molecular biology and applications

 

Theory

• UNIT I
• Model Systems in Plant Biology (Arabidopsis, Rice etc.) Forward and Reverse Genetic Approaches.
• UNIT II
• Organization expression and interaction of nuclear, mitochondrial and chloroplast genomes. Cytoplasmic male sterility.
• UNIT III
• Transcriptional and Post-transcriptional Regulation of Gene Expression, Isolation of promoters and other regulatory elements, RNA interference, Transcriptional Gene Silencing, Transcript and Protein Analysis.
• UNIT IV
• Plant Developmental Processes, ABC Model of Floral Development, Role of hormones (Ethylene, Cytokinin, Auxin and ABA, SA and JA) in plant development. Regulation of Flowering, Plant photoreceptors and light signal transduction, vernalization, Circadian Rhythms.
• UNIT V
• Abiotic Stress Responses: Salt, Cold, Heat and Drought.
• UNIT VI
• Biotic Stress Responses. Molecular Biology of Plant-pathogen Interactions, Molecular Biology of Rhizobium and Agrobacterium- Plant interaction. Role of programmed Cell Death in Development and Defense.

 

Suggested Readings

• Plant Biotechnology: The Genetic Manipulation of Plants (2008) by Adrian Slater, Nigel W. Scott, and Mark R. Fowler
• Plant Biotechnology and Genetics: Principles, Techniques and Applications (2008) by C. Neal Stewart Jr.
• Molecular biology and biotechnology (2009) by John M. Walker, Ralph Rapley Molecular biology: genes to proteins (2007) by Burton E. Tropp, David Freifelder Plant Molecular Biology by Buchanan et al.

 

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ADVANCES IN GENETIC ENGINEERING

Objective

• To provide in depth knowledge of recent developments in recombinant DNA and genetic engineering

 

Theory

• UNIT I
• General Overview of Transgenic Plants; Case studies: Genetic Engineering of Herbicide Resistance, Transgenic Plants Resistant to Insects/pests,
• UNIT II
• Genetic engineering of abiotic stress tolerance, Engineering Food Crops for Quality, Genetically engineered pollination control, Induction of male sterility in plants.
• UNIT III
• Molecular Farming of Plants for Applications in Veterinary and Human Medicine systems: Boosting heterologous protein production in transgenics, Rapid production of specific vaccines, High-yield production of therapeutic proteins in chloroplasts;
• UNIT IV
• Recent developments in plant transformation strategies; Role of RNAi-based gene silencing in crop improvement; Regulated and tissue-specific expression of transgenes for crop improvement; Gene stacking; Pathway Engineering; Marker-free transgenic development strategies; High throughput phenotyping of transgenic plants; Field studies with transgenic crops;
• UNIT V
• Environmental issues associated with transgenic crops; Food and feed safety issues associated with transgenic crops; Risk assessment of transgenic food crops.

 

Suggested Readings

• Principles of Gene Cloning (1994) by Old and Primrose
• Molecular biology of the cell (2007) by Bruce Alberts
• Principles of Gene Manipulation and Genomics by Sandy B. Primrose and Richard Twyman (Paperback - Feb. 17, 2006)

 

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ADVANCES IN MOLECULAR BREEDING AND GENOMICS  

Objective

• To provide in depth knowledge of recent developments in molecular breeding and genomics

 

Theory

• UNIT I
• Mapping genes and QTLs, statistical concepts in QTL mapping, high-throughput genotyping using automated platforms, genetic and physical mapping of genomes, study of population structure and kinship, association genetic analysis of QTL, case studies on QTL mapping using different approaches, map-based of cloning genes and QTLs – case studies
• UNIT II
• Marker assisted breeding (MAB): Principles and methods, marker assisted foreground and background selection, marker assisted recurrent selection, whole genome selection, case studies in MAS, requirement for successful marker assisted breeding, cost of MAB
• UNIT III
• Concepts and methods of next generation sequencing (NGS), assembly and annotation of NGS data, genome resequencing, DNA sequence comparison, annotation and gene prediction 
• Unit IV
• Genome-wide insertion mutagenesis and its use in functional genomics, transcriptome profiling using microarrays and deep sequencing, study of methylome and its significance, proteome analysis using mass spectrometry, crystallography and NMR, analysis of proteome data, study of proteinprotein interactions
• UNIT V
• Study of the metabolome, use of 1D/2D NMR and MS in metabolome analysis, multivariate analysis and identification of metabolite as biomarkers, study of ionome using inductively coupled plasma – mass spectroscopy (ICP-MS), correlating the data from genome, transcriptome, proteome, matabolome and ionome with phoneme.

 

Suggested Readings

• Proteome Analysis - David W. Speicher
• Metabolomics: The Frontier of Systems Biology - M. Tomita and T. Nishioka
• Molecular Biology and Genomics – C. Mulhardt
• Genomes – T. A. Brown
• Molecular Marker Systems in Plant Breeding and Crop Improvement - H. Lörz and G. Wenzel
• Statistical Genomics - B. H. Liu
• Plant Biotechnology and Genetics: Principles, Techniques and Applications (2008) by C. Neal Stewart Jr.
• Molecular biology and biotechnology (2009) by John M. Walker, Ralph Rapley
• Genomes (2006) by TA Brown
• New and Emerging Proteomic Techniques (2010) by D. Nedelkov and R. Nelson

 

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ADVANCES IN STRESS GENOMICS   

Objective

• To provide advanced knowledge on genomics with reference to abiotic and biotic stress resistance in plants

 

Theory

• UNIT I
• Stress resistance/tolerance genes from model plants such as Arabidopsis and rice, as well as from extremophiles; stress tolerance mechanisms.
• UNIT II
• Genomics: transcriptomes, small RNAs and epigenomes; functional genomics: transfer of resistance genes to model plants and validation of gene function
• Unit III
• Bioinformatics approaches to determine gene function and networks in model plants under stress

 

Suggested Readings

• Current Review articles and research papers